The Science behind DNA Portraits

DNA Art Gallery LLC has created a patent pending process for spectacularly producing DNA artwork using oligoclonal primers created to splice fragments of specific genes documented in scientific research documents to be associated with certain emotions or behaviors.  Using the polymerase chain reaction (PCR) technique, we then clone each of these isolated segments of DNA into millions of copies.  This amplification step is used to yield enough DNA required for analysis during the gel electrophoresis step, in which DNA fragments are separated based on size in an agarose gel.  Various restriction enzymes are additionally used to essentially "cut" the introns regions (non-coding) to create a unique DNA fingerprint.  During this step, the individual’s distinctive DNA fingerprint is created from specific gene fragments.  In general, the DNA sequence in 99.5% of all humans is identical.  However, every human genome is different because of mutations, or mistakes, that occur in a DNA sequence.  These changes, or mutations, accumulate in the intragenic regions much faster than the exon sequences.  The restriction enzymes splice the genomic fragments within the intragenic non-coding regions of the DNA, where the most variable sequences exist because they do not code for proteins.  The image of the individual’s unique DNA fingerprint is then captured as it fluoresces at a wavelength of 365 nanometers from the ethidium bromide stained gel using a UV transilluminator. Spectacular DNA Art Wall Painting


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